Composite

Part:BBa_K2481109:Design

Designed by: Erin Kelly   Group: iGEM17_Lethbridge_HS   (2017-10-27)


indC expression construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1311
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2574


Design Notes

The indC coding sequences was assembled with the T7 promoter wheich is induced by IPTG in E. coli BL21(DE3). We also included the E. coli RBS B0034 and terminators B0015.


Source

The original part (BBa_K1152008) was sourced from Photorhabdus luminescens laumondii TT01 genomic DNA. We had the DNA synthesized based on the sequence in the Parts Registry.

References